Journal: Cell Reports

Article Title: IgG targeting distinct seasonal coronavirus- conserved SARS-CoV-2 spike subdomains correlates with differential COVID-19 disease outcomes

doi: 10.1016/j.celrep.2022.110904

Figure Lengend Snippet:

Article Snippet: Human SARS coronavirus (SARS-CoV) Spike glycoprotein Gene ORF cDNA clone expression plasmid (Codon Optimized), C-Flag tag , SinoBiological , VG40150-CF.

Techniques: Virus, Recombinant, Cell Culture, Enzyme-linked Immunosorbent Assay, In Vitro, Transfection, Luciferase, Lysis, Expressing, Plasmid Preparation, Software, Sequencing

Journal: International Journal of Molecular Sciences

Article Title: The Functional Consequences of the Novel Ribosomal Pausing Site in SARS-CoV-2 Spike Glycoprotein RNA

doi: 10.3390/ijms22126490

Figure Lengend Snippet: Fragment of the multiple alignments of SARS-CoV-2 S protein RNAs: Sequences surrounding the CCTCGGCGGGCA insertion in the SARS-CoV-2 sequence (GenBank entry NC_045512.2, the SARS-CoV-2 reference sequence). MN996532.1 is the closest bat homolog RaTG13; MG772934.1, MG772933.1, and KT444582.1 are more distantly related bat homologs. Novel out-of-frame stop codons in the human SARS-CoV-2 are italicized.

Article Snippet: Based on the bioinformatic analyses, and to compare the expression of the original sequence spike protein and codon-optimized spike protein, we obtained the codon-optimized S protein cDNA construct (CCAAGGAGGGCA furin site, pCMV-codon-optimized spike, VG40589-UT, Sino Biologicals) and the original sequence cDNA construct (CCTCGGCGGGCA furin site, pUNO1-SARS2-S, InvivoGen).

Techniques: Sequencing

Journal: International Journal of Molecular Sciences

Article Title: The Functional Consequences of the Novel Ribosomal Pausing Site in SARS-CoV-2 Spike Glycoprotein RNA

doi: 10.3390/ijms22126490

Figure Lengend Snippet: Expression of S protein in pseudovirions expressing original and codon-optimized S proteins. Pseudovirions were produced in LV production cells, Expi293 cells, and adherent HEK293T cells. Upper panels were imaged at normal gain, while lower panels were imaged at higher gain to visualize original S protein expression (S original). Red asterisk indicates spike protein band.

Article Snippet: Based on the bioinformatic analyses, and to compare the expression of the original sequence spike protein and codon-optimized spike protein, we obtained the codon-optimized S protein cDNA construct (CCAAGGAGGGCA furin site, pCMV-codon-optimized spike, VG40589-UT, Sino Biologicals) and the original sequence cDNA construct (CCTCGGCGGGCA furin site, pUNO1-SARS2-S, InvivoGen).

Techniques: Expressing, Produced

Journal: Immunity

Article Title: Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses

doi: 10.1016/j.immuni.2020.11.015

Figure Lengend Snippet: Cross Reactivity of Nanoparticle Vaccine-induced Antibodies and T Cell Responses (A) Six BALB/c mice within each group were prime-boost-immunized with Ferritin core, HR monomer and HR-Ferritin nanoparticle, respectively. Serum was collected two weeks post boost vaccination and incubated with authentic SARS-CoV-2, followed by incubating with Vero E6 cells. The FRNTspots in 1:100 dilution were plotted for each group (n = 6). (B–F) Cross-neutralization of serum of immunized BALB/c was detected with pseudotyped-CoVs which contained SARS-CoV, MERS-CoV, HCoV-229E, HCoV-OC43, and RATG13 (n = 3). Neutralizations at dilution of 1: 30 of serum were shown. (G and H) Splenocytes of RBD and RBD-HR nanoparticle vaccines-immunized mice were incubated with hCoV-OC43 S peptides pool and hCoV-229E S peptides pool, respectively. ELISpot assay was conducted for IFN-γ secretion in splenocytes. Experiments were conducted independently in triplicates. Data represented as mean ± SEM. Adjusted p values in (A–F) were calculated by one-way ANOVA with Tukey’s multiple comparisons test. P values in (G) and (H) were calculated by Student’s t test. ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: HCoV-OC43 Spike Gene ORF cDNA clone expression plasmid , Sino Biological , Cat# VG40607-CF; GenBank: AVR40344.1.

Techniques: Incubation, Neutralization, Enzyme-linked Immunospot

Journal: Immunity

Article Title: Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses

doi: 10.1016/j.immuni.2020.11.015

Figure Lengend Snippet:

Article Snippet: HCoV-OC43 Spike Gene ORF cDNA clone expression plasmid , Sino Biological , Cat# VG40607-CF; GenBank: AVR40344.1.

Techniques: Purification, Recombinant, Enzyme-linked Immunosorbent Assay, RNA Detection, Enzyme-linked Immunospot, Bicinchoninic Acid Protein Assay, Luciferase, Transgenic Assay, Plasmid Preparation, Expressing, Software, Microscopy

Journal: Emerging Microbes & Infections

Article Title: A camel-derived MERS-CoV with a variant spike protein cleavage site and distinct fusion activation properties

doi: 10.1038/emi.2016.125

Figure Lengend Snippet: Comparison of predicted and measured furin-mediated proteolytic processing of EMC/2012 and HKU205 MERS-CoV S2′ sites

Article Snippet: Mammalian codon-optimized wild-type NRCE-HKU205 spike (205 wt , GenBank: AHL18090.1), and NRCE-HKU205 spike with S886A and I888S substitutions (205 EMC-S2 ′ ) containing C-terminal C9-epitope tag were synthesized (Biomatik, Wilmington, DE, USA) and subcloned in the pcDNA-3.1 vector.

Techniques: Comparison, Sequencing

Journal: Emerging Microbes & Infections

Article Title: A camel-derived MERS-CoV with a variant spike protein cleavage site and distinct fusion activation properties

doi: 10.1038/emi.2016.125

Figure Lengend Snippet: Summary of rates of proteolytic cleavage ( V max ) of EMC/2012 and HKU205 MERS-CoV S2′ sites

Article Snippet: Mammalian codon-optimized wild-type NRCE-HKU205 spike (205 wt , GenBank: AHL18090.1), and NRCE-HKU205 spike with S886A and I888S substitutions (205 EMC-S2 ′ ) containing C-terminal C9-epitope tag were synthesized (Biomatik, Wilmington, DE, USA) and subcloned in the pcDNA-3.1 vector.

Techniques: Control